|
|
Evaluation of endoglin and VCAM-1 expression and co-expression in aortas of apoE-deficient mice
Minaříková, Lucie ; Rathouská, Jana (advisor) ; Fikrová, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Evaluatiton of the expresion and co-expresion of endoglin and VCAM-1 in the aorta on apoE- deficient mice Diploma thesis Lucie Minaříková Supervisor: Mgr. Jana Rathouská Background: The aim of this work was to monitor the expression and a possible co-expression of endoglin (TGF-β receptor III) and VCAM-1 in mouse aortic endothelium. As an experimental model, we used the mouse strain C57BL/6J, that was genetically modified, and is characteristic by a deficit of apolipoprotein E. Methodes: In the study, we focused on testing the mouse strain C57BL/6J with gene knockout for apolipoprotein E in different stages of the atherosclerotic process. 10 weeks old female mice were divided into three groups and fed diets with different content of cholesterol. One experimental group was fed a standard diet (called "chow type diet") for a period of two months. The other two groups were fed a diet containing 21% fat (called "Western type diet") for a period of two and four months. For the determination of the levels of total cholesterol, a biochemical analysis of blood was performed. Obtained parts of ascending aorta were analyzed by ImmPRESSTM immunohistochemical method with the detection reagent DAB....
|
|
|
Pathophysiology of Spinal Cord Injury Studied by In Vivo Optical Imaging
Vančíková, Sabína ; Valášková, Barbora (advisor) ; Špicarová, Diana (referee)
Patients suffering from spinal cord injury experience physical, social, and vocational impairment. It is a condition often causing a permanent disability mainly due to axonal regeneration incapability in the central nervous system. The primary insult simultaneously damages cells in the lesion site and initiates a cascade of secondary cellular, vascular, and biochemical events extending the injury. These pathophysiological mechanisms are examined using multiple approaches. Novel imaging techniques complement classical histopathological methods and neuroanatomical tracing. Recent studies employ transgenic mice and two-photon microscopy to observe single cells in the injury site and the nearby vasculature in vivo longitudinally. In vivo optical imaging enables studying of axonal responses, such as degeneration, regeneration, and neurovascular interactions. It also gives an opportunity to assess the effects of applied drugs directly. New findings lead to a better understanding of the pathophysiology of spinal cord injury, resulting in the ability to develop other therapeutic strategies improving the outcome after injury. Keywords: spinal cord injury, pathophysiological mechanisms, axonal regeneration, Wallerian degeneration, animal models, transgenic mice, in vivo imaging, two-photon excitation microscopy
|
|
|
Evaluation of endoglin and VCAM-1 expression and co-expression in aortas of apoE-deficient mice
Minaříková, Lucie ; Rathouská, Jana (advisor) ; Fikrová, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Evaluatiton of the expresion and co-expresion of endoglin and VCAM-1 in the aorta on apoE- deficient mice Diploma thesis Lucie Minaříková Supervisor: Mgr. Jana Rathouská Background: The aim of this work was to monitor the expression and a possible co-expression of endoglin (TGF-β receptor III) and VCAM-1 in mouse aortic endothelium. As an experimental model, we used the mouse strain C57BL/6J, that was genetically modified, and is characteristic by a deficit of apolipoprotein E. Methodes: In the study, we focused on testing the mouse strain C57BL/6J with gene knockout for apolipoprotein E in different stages of the atherosclerotic process. 10 weeks old female mice were divided into three groups and fed diets with different content of cholesterol. One experimental group was fed a standard diet (called "chow type diet") for a period of two months. The other two groups were fed a diet containing 21% fat (called "Western type diet") for a period of two and four months. For the determination of the levels of total cholesterol, a biochemical analysis of blood was performed. Obtained parts of ascending aorta were analyzed by ImmPRESSTM immunohistochemical method with the detection reagent DAB....
|
|
|
Differentiation potential of polydendrocytes after focal cerebral ischemia
Filipová, Marcela ; Anděrová, Miroslava (advisor) ; Jendelová, Pavla (referee)
Ischemic injury leeds to sequence of pathophysiological events, which are accompanied by a release of growth factors and morphogens that significantly affect cell proliferation, migration and also their differentiation. Following ischemia, besides enhanced neurogenesis and gliogenesis in subventricular zone of the lateral ventricles and gyrus dentatus of the hippocampus, neurogenesis/gliogenesis also occurs in non-neurogenic regions, such as cortex or striatum. Recently, the attention was turned to a new glial cell type, termed polydendrocytes or NG2 glia. Under physiological conditions, these cells are able to divide and differentiate into mature oligodendrocytes due to they have often been equated with oligodendrocyte precursor cells. Based on recent reports, polydendrocytes are also able to generate protoplasmic astrocytes (Zhu et al., 2008) and neurons in vitro (Belachew et al., 2003), however their ability to differentiate into astrocytes or neurons under physiological or pathological conditions is still highly debated. Therefore, we have investigated the effect of different growth factors and morphogens, specifically brain-derived neurotrophic factor (BDNF), basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF) and a morphogen sonic hedgehog (Shh), on...
|
guest ::
